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For all interactions, 16 serially diluted titrations of suramin (concentration ranging from 30 nM to 1 mM) were mixed with a fixed concentration (50 nM) of the eGFP-labeled polymerases in the buffer containing 20 mM HEPES, pH 7.5, either 150 or 500 mM NaCl, 1 mM TCEP and 0.05% Tween 20